Any reference genes and control assays and controls can also be added.
The custom builder allows you to upload your own list of targets and add any predesigned or custom assays of your choice. QuantiNova LNA Probe PCR Custom Panels are a fully customizable product and are available in 96-well plate, 384-well plate and 100-well disc formats, with all of our different plate layouts available. Assays that do not span an intron are designated as such, and if there is one exon in the target, unwanted signals can be easily eliminated using the QuantiNova Reverse Transcription Kit with the integrated gDNA removal step. Most of the assays are intron-spanning when possible and detect only RNA. The predesigned assays cover most transcripts in the Ensembl database for human, mouse and rat genes, enabling PCR-based gene expression studies in the greatest depth possible. Our proprietary algorithm has been used to design over 1.3 million QuantiNova LNA Probe PCR Assays to provide the most sensitive, accurate and effective mRNA and lncRNA analysis. However, we’ve already optimized the QuantiNova LNA Probe PCR system for you to eliminate non-specific amplification and give you accurate and sensitive detection every time. Probe-based detection still requires high PCR specificity to prevent amplification artifacts such as non-specific PCR products and primer–dimers that can compete for reaction components and compromise performance. QuantiNova LNA Probe PCR Custom Panels use FAM-labeled hydrolysis probe-based detection, which is highly sensitive and robust, because only the desired PCR product is detected. Robust and high-performance hydrolysis probe-based qPCR This gives you robust and reliable quantification, even for AU-rich targets, low-abundance transcripts, targets with high secondary structure and highly complex samples. The high binding affinity of LNA bases increases the flexibility of primer and probe placement on the transcript, so we are able to use intelligent positioning to design assays for otherwise difficult-to-analyze targets. Increased specificity from the clever placement of LNA gives you a high signal-to-noise ratio, allowing discrimination of sequences that differ by only a single nucleotide and eliminating non-specific amplification and primer-dimer formation. The increased sensitivity ensures excellent amplification efficiencies down to 1 RNA molecule, making it easier for you to detect low-abundance targets such as lncRNAs from less starting material (see figure QuantiNova LNA Probe PCR Assays provide accurate, sensitive and linear quantification of targets over a wide dynamic range and figure QuantiNova LNA Probe PCR Assays enable both high-expression and low-expression detection of mRNA). LNA enhancement enables T m normalization across the panel giving the primers and probes higher binding affinity, dramatically increasing assay sensitivity and specificity. Each primer–probe set delivers the highest specificity and efficiency for the most reliable and accurate results. Twenty years of LNA design experience has enabled us to optimize our sophisticated LNA design algorithm, which incorporates over 50 different parameters to guarantee the most optimal assay for successful target detection. Expert LNA enhancement for top qPCR performanceĪll QuantiNova LNA Probe PCR Assays are developed using stringent design criteria and lab-validated algorithms.
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